Chromatographic methods for the detection, purification and separation of Dendrimers

Abstract

Dendrimers have aroused scientific temper since their first hypothesis by Flory and later reported by Fréchet and co – workers. Properties such as pharmacodynamics and bio distribution of these unique polymers are dependent on their size and diffusion rates. Reverse Phase High Performance Liquid Chromatography (RP – HPLC) and Ultra High Pressure Liquid Chromatography (UPLC) are two unique methods used in the purification and quantification of various types of dendrimers. RP – HPLC is usually the primary method for usual sample analysis, being more rugged in comparison to various other forms of liquid chromatography. Columns for RP – HPLC and UPLC are non – polar, stable and reproducible. Though UP – HPLC offers less run time and increases sensitivity, there are associated problems with reduction in column life due to decreased particle size and high pressure. The present review serves to examine methodologies in the use of both these separation techniques in the analyses of dendrimers.

Keywords

Dendrimers, RP – HPLC, UPLC

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