In this study, we examined the medicinal properties of medicinal plants Achyranthes aspera Linn and Jatropha gossypifolia individually as well as their synergism. Different solvents system used to extract the principle compound present in these selected plants and solvent were chosen based on their polarity from high polar solvent to low polar. The ethanol extract of Achyranthes aspera and Jatropha gossypifolia has maximum activity against the microorganism Staphylococcus aureus. The effective compounds present in the extracted sample were evaluated by generating chromatogram in Thin Layer Chromatography technique. The quality and purity were confirmed by HPLC. The synergistic activity of ethanolic extract of these plants shows significant antibacterial activity.