Acute phase protein synthesis and the production of neutrophils in the bone marrow was depend on the cytokine named as IL-6 which is a Powerful Pro-inflammatory cytokine. It also supports the growth of B cells and is antagonistic to regulatory T cells. The aim of the present study was to investigate the S.oralis mediated IL-6 production in cord blood derived human stem cells (CBSC) in comparison with peripheral blood mononuclear cells (PBMC). In this study, stem cells were isolated from the umbilical cord blood and mononuclear cells were isolated from pheripheral blood of a healthy donar. A virulent heat inactivated Streptococcus oralis bacterial culture was used to stimulate both cord blood derived human stem cells (CBSC) in comparison with peripheral blood mononuclear cells (PBMC). After stimulation the culture supernatant was collected and subjected to quantification of IL-6 release by ELISA. The results showed that the Streptococcus oralis induces the IL-6 cytokine release in PBMC cells than the cord blood derived human stem cells.