STRAIN IMPROVEMENT FOR AMYLASE PRODUCTION BY INDUCED MUTATION

Abstract

Mutations are changes in the nucleotide sequence of an organism's genetic material. Mutations can be caused by copying errors in the genetic material during cell division, exposure to ultraviolet or ionizing radiation, chemical mutations, or viruses, or by cellular processes, such as hyper mutation. In multicellular organisms with dedicated reproductive cells, mutations can be divided into germ line mutations, which can be passed on to offspring via reproductive cells, and somatic mutations, which involve cells outside a specific reproductive group and are not usually transmitted to offspring. In this work the organism producing amylase was isolated from the soil and identified based on morphological and biochemical experiments.Temperature of various carbon and nitrogen sources, pH changes in amylase production. By using UV, edium bromide and nitrosoquanidine the organism becomes mutated and the amylase production is checked, the genetic DNA mutation is checked and the amylase-producing genes are amplified by PCR.