ENHANCING OF β-GALACTOSIDASE PRODUCTION FROM ASPERGILLUS SPECIES BY INDUCED MUTAGENESIS UNDER SOLID-STATE FERMENTATION

Abstract

The first antibiotic was discovered in 1896 by Ernest Duchesne and "rediscovered" by Alexander Flamming in 1928 from the filamentous fungus Penicilium notatum, Antibiotics can be selectively toxic by targeting such features as the bacterial cell wall. In present study the Penicillum species was isolated from soil sample through serial dilution and plating method and the genus was identified by wet-mount technique after that the fungi .The Penicillumsp was grown in the growth medium , after mat formation it was transferred in to production medium and the production was conformed by estimating the enzyme by DNS method. The same way the Penicillium sp was subjected to mutation by UV irradiation and Ethidioum Bromide and it was compared with wiled type. In that compere with wiled type I got the better production in UV mutation, in Ethidium Bromide mutation , the production was reduced so the to conclude my work the UV mutant strain was produced better result in production of Penicillinase enzyme. In future i will go for mass production of the enzyme and reduce the cost of the Antibiotic.