L ASPARAGINASE CHARACTERIZATION AND NANO ENCAPSULATION

Abstract

Halophilic bacteria are organisms which inhabit the salt-rich environment were isolated and characterized by conventional and molecular methods. Sediment samples were collected from four thoothukudi salt pan, Tamil Nadu. Totally, 4 halophilic bacteria were isolated and their colony characteristics were recorded. The results of biochemical tests are in accordance with Bacillus sp. Halobacterium salinarum , E.coli and Staphylococcus citreus were identified based on the morphological, physiological and biochemical characterization.. In this present study, we have screened moderate Halophilic bacteria for L-asparaginase production ability and showed that intra- and extracellular L-asparaginas was produced by Halophilic bacteria. The isolate T2 identified as Halobacterium salinarum only strain found to L asparaginase producer and enzyme assay was increased 34U to 82 U followed by immobilization with alginate and chitosan mixture. The total protein was also recorded higher in immobilized cell culture. It concludes that chitosan was best natural polymer effectively entrap the cells either alone or with alginate and improve the efficiency of cells.
Key words: Halobacterium salinarum, L Asparaginase, Immobilization,