Preparation of Polyherbal Formulation for Common Cold and Toxicity Analysis in Zebra Fish Model

Abstract

Polypharmacy often referred as Polyherbalism is the combination of several medicinal herbs to achieve therapeutic effectiveness. Polyherbalism tends to provide larger benefits in a synergistic interaction. This investigation aims to study the phytochemical characterization of Vapcozam polyhedral formulation extract, and their anti-inflammatory and anti-oxidant activity in in-vitro condition followed by toxicity analysis using the zebra fish model. Thepolyherbal formulation was prepared from tendifferent plant materials using Soxhlet apparatus. The phytochemical characterization of Vapcozam polyhedral formulation was carried out and their anti-inflammatory activity was attained by the albumin denaturation assay.The anti-oxidant activity of polyhedral formulation was studied using the hydrogen peroxide assay.A dose response curve was plotted to determine the IC50 values using graph pad prism software. The traditional medicinal poly herbal plants formulation gave promising results for the treatment of cough and common cold. Phytochemical characterization results confirmed the presence of tannins, flavanoids, carbohydrates, and glycosides obtained from the Vapcozam polyhedral formulation. A maximum anti-inflammatory activity was obtained at 46.79μg/ml. The antioxidant results showed 117 μg/ml of IC50 value using hydrogen peroxide assay. Toxicity analysis using zebra fish model highlighted the efficacy of the drug usage for the treatment of common cold. Taken together, these results showed that the Vapcozam polyhedral formulation could be a effective treatment method for common cold.