EFFECT OF ALOE VERA EXTRACT ON SOME BIOCHEMICAL MARKERS OF ENDOSULFAN TOXICITY IN CYPRINUS CARPIO
Abstract
Endosulfan is a persistent organic pollutant especially in water bodies and sediments with increased danger of bioaccumulation. Although it was banned worldwide by the Stockholm Convention in 2011, it is still used especially in developing countries. It is extremely harmful to non-target organisms in aquatic bodies especially fishes. In the present study, effect of Aloe vera on some specific bio chemical marker enzymes of endosulfan intoxicated common carp, Cyprinus carpio was investigated. Test animals were exposed to sublethal concentrations (0.05µg/L) of the endosulfan and Aloe vera was given in feed at two different concentrations (10% and 20% w/w). The preventive and curative effect of Aloe vera was determined by studying the level of marker enzymes like acetyl cholinesterase in brain, superoxide dismutase in liver and acid and alkaline phosphatases in muscle. The total protein content in all these samples was also estimated. It was found that Aloe vera has significant preventive effect in combating the toxicity of endosulfan. The enzyme systems that get disrupted in endosulfan treated groups showed an improvement in their activity in Aloe vera feed given preventive groups. Even though lesser when compared to the preventive effect, Aloe vera also showed a significant curative effect in the endosulfan damaged enzyme systems but only in the higher concentration. All the results obtained were found to be statistically significant (p < 0.05).
Keywords
Endosulfan, Aloe vera, Acetyl cholinesterase, Superoxide dismutase, Alkaline phosphatase, Cyprinus carpio
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