The field of nanotechnology is the most active area of research in modern materialsscience. Though there are many chemical as well as physical methods, green synthesis ofnanomaterials is the most emerging method of synthesis. We report the synthesis ofantibacterial silver nanoparticles (AgNPs) using leaf broth of medicinal herb, Ocimumsanctum (Thulasi). The synthesized AgNPs have been characterized by UV-Vis spectroscopy,transmission electron microscopy (TEM), and X-ray diffractometry. The mean particle ofsynthesized NPs was found to be 18 nm, as confirmed by TEM. The qualitative assessment ofreducing potential of leaf extract has also been carried out which indicated presence ofsignificant amount of reducing entities. FTIR analysis revealed that the AgNPs werestabilized by eugenols, terpenes, and other aromatic compounds present in the extract.